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mouse antigfp tag  (Thermo Fisher)


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    Thermo Fisher mouse antigfp tag
    Mouse Antigfp Tag, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse antigfp tag/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    mouse antigfp tag - by Bioz Stars, 2026-06
    90/100 stars

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    PHEV infection leads to lysosomal abnormalities. a PHEV localizes to lysosomes, and PHEV infection results in greater lysosome enlargement compared with mock infection. The percentage of N2a cells containing enlarged lysosomes (> 1 μm) was quantified in the experiment. Scale bar = 10 μm. All the results are presented as the means ± the SD of the data from three independent experiments (**, P < 0.01). b PHEV infection decreased PGRN protein expression. PGRN protein levels in PHEV-infected or mock-infected N2a cells were quantified and normalized to GAPDH. n = 3; one-way ANOVA; **, P < 0.01; Student’s t test. c PHEV infection decreased PGRN mRNA expression. PGRN mRNA levels in PHEV-infected or mock-infected N2a cells were quantified and normalized to GAPDH. n = 3; one-way ANOVA; **, P < 0.01; Student’s t test. d PHEV bound to PGRN. PHEV-infected or mock-infected N2a cells were stained with antiPHEV (red) and antiPGRN (green). Scale bar = 10 μm. e AntiGFP immunoprecipitates from PHEV-infected or mock-infected EGFP-PGRN-transfected N2a cells were harvested, and the physical interaction between PGRN and PHEV was demonstrated

    Journal: Molecular Neurobiology

    Article Title: An Experimental Model of Neurodegenerative Disease Based on Porcine Hemagglutinating Encephalomyelitis Virus–Related Lysosomal Abnormalities

    doi: 10.1007/s12035-020-02105-y

    Figure Lengend Snippet: PHEV infection leads to lysosomal abnormalities. a PHEV localizes to lysosomes, and PHEV infection results in greater lysosome enlargement compared with mock infection. The percentage of N2a cells containing enlarged lysosomes (> 1 μm) was quantified in the experiment. Scale bar = 10 μm. All the results are presented as the means ± the SD of the data from three independent experiments (**, P < 0.01). b PHEV infection decreased PGRN protein expression. PGRN protein levels in PHEV-infected or mock-infected N2a cells were quantified and normalized to GAPDH. n = 3; one-way ANOVA; **, P < 0.01; Student’s t test. c PHEV infection decreased PGRN mRNA expression. PGRN mRNA levels in PHEV-infected or mock-infected N2a cells were quantified and normalized to GAPDH. n = 3; one-way ANOVA; **, P < 0.01; Student’s t test. d PHEV bound to PGRN. PHEV-infected or mock-infected N2a cells were stained with antiPHEV (red) and antiPGRN (green). Scale bar = 10 μm. e AntiGFP immunoprecipitates from PHEV-infected or mock-infected EGFP-PGRN-transfected N2a cells were harvested, and the physical interaction between PGRN and PHEV was demonstrated

    Article Snippet: The following primary antibodies were used in this study: rat antimouse LAMP1 antibody (553792) from BD Biosciences, sheep antiGRN/progranulin antibodies from R&D systems (AF2557), rabbit antiTDP-43 antibodies (0782-2-AP), rabbit antiCTSD antibodies (21327-1-AP), and mouse antiGFP antibodies (66002-1-Ig) from Proteintech.

    Techniques: Infection, Expressing, Staining, Transfection